Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.

ABSTRACT:

Background

Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease affecting the axial skeleton and peripheral joints. The etiology of this disease remains poorly understood, but interactions between genetic and environmental factors have been implicated. The present study identified differentially expressed proteins in the synovial fluid (SF) of AS patients to elucidate the underlying cause of AS.

Methods

A cohort of 40 SF samples from 10 AS and 10 each of rheumatoid arthritis (RA), gout, and osteoarthritis (OA) patients were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins specific to AS. The label-free LC-MS/MS results were verified by western blotting.

Results

We identified 8 protei

SUBMITTER: Lee JH 

PROVIDER: S-EPMC7269004 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature