Quantitative Proteomics of All 14 Renal Tubule Segments in Rat.

ABSTRACT:

Background

Previous research has used RNA sequencing in microdissected kidney tubules or single cells isolated from the kidney to profile gene expression in each type of kidney tubule epithelial cell. However, because proteins, not mRNA molecules, mediate most cellular functions, it is desirable to know the identity and amounts of each protein species to understand function. Recent improvements in the sensitivity of mass spectrometers offered us the ability to quantify the proteins expressed in each of 14 different renal tubule segments from rat.

Methods

We manually dissected kidney tubules from rat kidneys and subjected samples to protein mass spectrometry. We used the "proteomic ruler" technique to estimate the number of molecules of each protein per cell.

Results

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SUBMITTER: Limbutara K 

PROVIDER: S-EPMC7269347 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature