Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.

ABSTRACT: Applying digital PCR (dPCR) technology to challenging clinical and industrial detection tasks has become more prevalent because of its capability for absolute quantification and rare target detection. However, practices learned from quantitative PCR (qPCR) that promote assay robustness and wide-ranging utility are not readily applied in dPCR. These include internal amplification controls to account for false-negative reactions and amplicon high-resolution melt (HRM) analysis to distinguish true positives from false positives. Incorporation of internal amplification controls in dPCR is challenging because of the limited fluorescence channels available on most machines, and the application of HRM analysis is hindered by the separation of heating and imaging functions on most dPCR systems. We

SUBMITTER: Aralar A 

PROVIDER: S-EPMC7269394 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature