Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos.

ABSTRACT: Genetic transformation is one of the most important technologies for revealing or modulating gene function. It is used widely in both functional genomics and molecular breeding of rice. Demands on its use in wild Oryza species is increasing because of their high genetic diversity. Given the difficulties in genetic crosses between distantly related species, genetic transformation offers a way to alter or transfer genetic traits in wild rice accessions. However, transformation of wild Oryza accessions by conventional methods using calli induced from scutellum tissue of embryos in mature seeds often fails. Here, we report methods using immature embryos for the genetic transformation of a broad range of Oryza species. First, we investigated the ability of callus induction and regeneration from

SUBMITTER: Shimizu-Sato S 

PROVIDER: S-EPMC7270233 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature