Selective Maleylation-Directed Isobaric Peptide Termini Labeling for Accurate Proteome Quantification.

ABSTRACT: Isobaric peptide termini labeling (IPTL) is an attractive protein quantification method because it provides more accurate and reliable quantification information than traditional isobaric labeling methods (e.g., TMT and iTRAQ) by making use of the entire fragment-ion series instead of only a single reporter ion. The multiplexing capacity of published IPTL implementations is, however, limited to three. Here, we present a selective maleylation-directed isobaric peptide termini labeling (SMD-IPTL) approach for quantitative proteomics of LysC protein digestion. SMD-IPTL extends the multiplexing capacity to 4-plex with the potential for higher levels of multiplexing using commercially available ¹³C/¹⁵N labeled amino acids. SMD-IPTL is achieved in a one-pot reaction in thre

SUBMITTER: Tian X 

PROVIDER: S-EPMC7271076 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature