HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

ABSTRACT:

Objective

To discover the correlated gene with HEATR1 in regulating chemoresistance of gemcitabine.

Methods

Gene chip analysis was performed to find out differential genes between HEATR1-KD and control groups. The top 20 genes were subjected to high-content screening, and functional assay was implemented. Gene expression profiling was carried out to find the downstream target. Immunohistochemistry and survival analysis were performed.

Results

ZNF185 fold change (4.5285) was the most significant between the HEATR1-KD and control groups. Knocking down ZNF185 could promote the chemosensitivity, apoptosis, and proliferative inhibition, with SMAD4 significantly upregulated. Patients with high HEATR1 and SMAD4 or low ZNF185 exhibited better survival.

Conclusion

HEATR

SUBMITTER: Fang Y 

PROVIDER: S-EPMC7271247 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature