ZSCAN4⁺ mouse embryonic stem cells have an oxidative and flexible metabolic profile.

ABSTRACT: Cultured mouse embryonic stem cells are a heterogeneous population with diverse differentiation potential. In particular, the subpopulation marked by Zscan4 expression has high stem cell potency and shares with 2 cell stage preimplantation embryos both genetic and epigenetic mechanisms that orchestrate zygotic genome activation. Although embryonic de novo genome activation is known to rely on metabolites, a more extensive metabolic characterization is missing. Here we analyze the Zscan4⁺ mouse stem cell metabolic phenotype associated with pluripotency maintenance and cell reprogramming. We show that Zscan4⁺ cells have an oxidative and adaptable metabolism, which, on one hand, fuels a high bioenergetic demand and, on the other hand, provides intermediate metabolites for epigenetic reprogramming. Our findings enhance our understanding of the metastable Zscan4⁺ stem cell state with potential applications in regenerative medicine.

SUBMITTER: Troiano A 

PROVIDER: S-EPMC7271321 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature