Exploration of anti-inflammatory mechanism of forsythiaside A and forsythiaside B in CuSO₄-induced inflammation in zebrafish by metabolomic and proteomic analyses.

ABSTRACT:

Background

Inflammation is a general pathological phenomenon during severe disturbances to the homeostasis. Forsythiaside A (FA) and forsythiaside B (FB), isolated from the dried fruit of Forsythia suspensa (Thunb.) Vahl, are phenylethanoid compounds that show a significant anti-inflammatory effect. However, the properties and therapeutic mechanisms of this effect have not yet been systematically elucidated.

Methods

In this study, the anti-inflammatory effects of FA and FB were investigated in CuSO₄-induced inflammation in zebrafish larvae. Intracellular generation of reactive oxygen species (ROS) and nitric oxide (NO) was investigated using fluorescence probes. Metabolomic and proteomic analyses using liquid chromatography-mass spectrometry were carried out to id

SUBMITTER: Gong L 

PROVIDER: S-EPMC7271515 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature