Genetically Targeted Optical Control of an Endogenous G Protein-Coupled Receptor.

ABSTRACT: G protein-coupled receptors (GPCRs) are membrane proteins that play important roles in biology. However, our understanding of their function in complex living systems is limited because we lack tools that can target individual receptors with sufficient precision. State-of-the-art approaches, including DREADDs, optoXRs, and PORTL gated-receptors, control GPCR signaling with molecular, cell type, and temporal specificity. Nonetheless, these tools are based on engineered non-native proteins that may (i) express at nonphysiological levels, (ii) localize and turnover incorrectly, and/or (iii) fail to interact with endogenous partners. Alternatively, membrane-anchored ligands (t-toxins, DARTs) target endogenous receptors with molecular and cell type specificity but cannot be turned on and off. I

SUBMITTER: Donthamsetti PC 

PROVIDER: S-EPMC7271769 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature