A cost-effective approach to DNA methylation detection by Methyl Sensitive DArT sequencing.

ABSTRACT: Several studies suggest the relation of DNA methylation to diseases in humans and important phenotypes in plants drawing attention to this epigenetic mark as an important source of variability. In the last decades, several methodologies were developed to assess the methylation state of a genome. However, there is still a lack of affordable and precise methods for genome wide analysis in large sample size studies. Methyl sensitive double digestion MS-DArT sequencing method emerges as a promising alternative for methylation profiling. We developed a computational pipeline for the identification of DNA methylation using MS-DArT-seq data and carried out a pilot study using the Eucalyptus grandis tree sequenced for the species reference genome. Using a statistic framework as in differential exp

SUBMITTER: Pereira WJ 

PROVIDER: S-EPMC7272069 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature