Neural tube development depends on notochord-derived sonic hedgehog released into the sclerotome.

ABSTRACT: Sonic hedgehog (Shh), produced in the notochord and floor plate, is necessary for both neural and mesodermal development. To reach the myotome, Shh has to traverse the sclerotome and a reduction of sclerotomal Shh affects myotome differentiation. By investigating loss and gain of Shh function, and floor-plate deletions, we report that sclerotomal Shh is also necessary for neural tube development. Reducing the amount of Shh in the sclerotome using a membrane-tethered hedgehog-interacting protein or Patched1, but not dominant active Patched, decreased the number of Olig2⁺ motoneuron progenitors and Hb9⁺ motoneurons without a significant effect on cell survival or proliferation. These effects were a specific and direct consequence of Shh reduction in the mesoderm. In add

SUBMITTER: Kahane N 

PROVIDER: S-EPMC7272346 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature