Analysis of human metabolism by reducing the complexity of the genome-scale models using redHUMAN.

ABSTRACT: Altered metabolism is associated with many human diseases. Human genome-scale metabolic models (GEMs) were reconstructed within systems biology to study the biochemistry occurring in human cells. However, the complexity of these networks hinders a consistent and concise physiological representation. We present here redHUMAN, a workflow for reconstructing reduced models that focus on parts of the metabolism relevant to a specific physiology using the recently established methods redGEM and lumpGEM. The reductions include the thermodynamic properties of compounds and reactions guaranteeing the consistency of predictions with the bioenergetics of the cell. We introduce a method (redGEMX) to incorporate the pathways used by cells to adapt to the medium. We provide the thermodynamic curation of

SUBMITTER: Masid M 

PROVIDER: S-EPMC7272419 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature