Single platelet variability governs population sensitivity and initiates intrinsic heterotypic responses.

ABSTRACT: Investigations into the nature of platelet functional variety and consequences for homeostasis require new methods for resolving single platelet phenotypes. Here we combine droplet microfluidics with flow cytometry for high throughput single platelet function analysis. A large-scale sensitivity continuum was shown to be a general feature of human platelets from individual donors, with hypersensitive platelets coordinating significant sensitivity gains in bulk platelet populations and shown to direct aggregation in droplet-confined minimal platelet systems. Sensitivity gains scaled with agonist potency (convulxin > TRAP-14>ADP) and reduced the collagen and thrombin activation threshold required for platelet population polarization into pro-aggregatory and pro-coagulant states. The heterotyp

SUBMITTER: Jongen MSA 

PROVIDER: S-EPMC7272428 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature