MAPS-seq: magnetic bead-assisted parallel single-cell gene expression profiling.

ABSTRACT: Recently developed single-cell RNA sequencing methods allow the simultaneous profiling of the transcriptomes of thousands of individual cells. However, current methods still require advanced equipment or entail substantial waste of reagents. Here, we introduce magnetic bead-assisted parallel single-cell gene expression sequencing (MAPS-seq), a microwell-based method that pools samples before the reverse transcription step, increasing the ease of sample preparation and reducing reagent waste. Moreover, because this method uses universal reagents and standard molecular biology lab instruments, it is easy to implement, even in labs that have not previously conducted single-cell RNA sequencing. We validated our method by demonstrating that it can generate gene expression data at the single-cel

SUBMITTER: Park M 

PROVIDER: S-EPMC7272464 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature