Mitogen-activated protein kinase regulation of the phosphodiesterase RegA in early Dictyostelium development.

ABSTRACT: Mitogen-activated protein kinase (MAPK) regulation of cAMP-specific phosphodiesterase function has been demonstrated in mammalian cells and suspected to occur in other eukaryotes. Epistasis analysis in the soil amoeba Dictyostelium discoideum suggests the atypical MAPK Erk2 downregulates the function of the cAMP-specific phosphodiesterase RegA to regulate progression of the developmental life cycle. A putative MAPK docking motif located near a predicted MAPK phosphorylation site was characterized for contributions to RegA function and binding to Erk2 because a similar docking motif has been previously characterized in the mammalian PDE4D phosphodiesterase. The overexpression of RegA with alterations to this docking motif (RegA^D-) restored RegA function to regA- cel

SUBMITTER: Adhikari N 

PROVIDER: S-EPMC7273326 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature