Human interleukin-4-treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model.

ABSTRACT: Murine alternatively activated macrophages can exert anti-inflammatory effects. We sought to determine if IL-4-treated human macrophages [i.e., hM(IL4)] would promote epithelial wound repair and can serve as a cell transfer treatment for inflammatory bowel disease (IBD). Blood monocytes from healthy volunteers and patients with active and inactive IBD were converted to hM(IL4)s. IL-4 treatment of blood-derived macrophages from healthy volunteers and patients with inactive IBD resulted in a characteristic CD206⁺CCL18⁺CD14^low/- phenotype (RNA-seq revealed IL-4 affected expression of 996 genes). Conditioned media from freshly generated or cryopreserved hM(IL4)s promoted epithelial wound healing in part by TGF, and reduced cytokine-driven loss of epithelial bar

SUBMITTER: Jayme TS 

PROVIDER: S-EPMC7274799 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature