MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma.

ABSTRACT:

Background

Metastasis-associated in colon cancer 1 (MACC1) is an established marker for metastasis and tumor cell migration in a multitude of tumor entities, including glioblastoma (GBM). Nevertheless, the mechanism underlying the increased migratory capacity in GBM is not comprehensively explored.

Methods

We performed live cell and atomic force microscopy measurements to assess cell migration and mechanical properties of MACC1 overexpressing GBM cells. We quantified MACC1 dependent dynamics of 3D aggregate formation. For mechanistic studies we measured the expression of key adhesion molecules using qRT-PCR, and MACC1 dependent changes in short term adhesion to fibronectin and laminin. We then determined changes in sub-cellular distribution of integrins and actin in dependen

SUBMITTER: Hohmann T 

PROVIDER: S-EPMC7275321 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature