Comparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell types.

ABSTRACT:

Background

Red blood cell (RBC) polymorphisms are suggested to influence the course of Plasmodium falciparum malaria. Whereas some variants have been found to be protective, others have been found to enhance parasite development. This study evaluated the effect of variant haemoglobin (Hb) and ABO blood groups on P. falciparum merozoite invasion, multiplication rates as well as gametocyte development.

Methods

Approximately 2.5 mL of venous blood was collected from each participant. Flow cytometry was used to determine the in vitro merozoite invasion rates of NF54 parasites into the blood of 66 non-parasitaemic individuals with variant Hb genotypes (HbSS, HbSC) and blood groups (A, B, O), which were then compared with invasion into HbAA blood. The ex vivo asexual parasite mult

SUBMITTER: Amoah LE 

PROVIDER: S-EPMC7275330 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature