Prognostic value of OCT4A and SPP1C transcript variant co-expression in early-stage lung adenocarcinoma.

ABSTRACT:

Background

Octamer-binding transcription factor 4A (OCT4A) is essential for cell pluripotency and reprogramming both in humans and mice. To date, however, the function of human OCT4 in somatic and/or tumour tissues is largely unknown.

Methods

RT-PCR was used to identify full-length splice forms of OCT4 transcripts in normal and cancer cells. A FLAG-tagged OCT4 genomic transgene was used to identify OCT4-positive cancer cells. A potential role for OCT4 in somatic cancer cells was examined by cell ablation of OCT4-positive cells using promoter-driven diphtheria toxin A. OCT4 and secreted phosphoprotein 1 (SPP1) transcripts in early-stage lung adenocarcinoma tumours were analysed and compared with pathohistological features.

Results

The results show that, unlike in murin

SUBMITTER: Koshimune S 

PROVIDER: S-EPMC7275395 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature