Th17 cells inhibit CD8⁺ T cell migration by systematically downregulating CXCR3 expression via IL-17A/STAT3 in advanced-stage colorectal cancer patients.

ABSTRACT:

Background

CD8⁺ T cell trafficking to the tumor site is essential for effective colorectal cancer (CRC) immunotherapy. However, the mechanism underlying CD8⁺ T cell infiltration in colorectal tumor tissues is not fully understood. In the present study, we investigated CD8⁺ T cell infiltration in CRC tissues and the role of chemokine-chemokine receptor signaling in regulation of T cell recruitment.

Methods

We screened chemokines and cytokines in healthy donor and CRC tissues from early- and advanced-stage patients using multiplex assays and PCR screening. We also utilized transcription factor activation profiling arrays and established a xenograft mouse model.

Results

Compared with tumor tissues of early-stage CRC patients, CD8⁺

SUBMITTER: Wang D 

PROVIDER: S-EPMC7275425 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature