Production and characterization of avian crypt-villus enteroids and the effect of chemicals.

ABSTRACT:

Background

Three-dimensional models of cell culture such as organoids and mini organs accord better advantage over regular cell culture because of their ability to simulate organ functions hence, used for disease modeling, metabolic research, and the development of therapeutics strategies. However, most advances in this area are limited to mammalian species with little progress in others such as poultry where it can be deployed to study problems of agricultural importance. In the course of enterocyte culture in chicken, we observed that intestinal mucosal villus-crypts self-repair and form spheroid-like structures which appear to be useful as ex vivo models to study enteric physiology and diseases.

Results

The villus-crypts harvested from chicken intestinal mucosa were cultu

SUBMITTER: Acharya M 

PROVIDER: S-EPMC7275437 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature