Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants.

ABSTRACT:

Background

Generation of marker-free transgenic plants is very important to the regulatory permission and commercial release of transgenic crops. Co-transformation methods that enable the removal of selectable marker genes have been extensively used because they are simple and clean. Few comparisons are currently available between different strain/plasmid co-transformation systems, and also data are related to variation in co-transformation frequencies caused by other details of the vector design.

Results

In this study, we constructed three vector systems for the co-transformation of allotetraploid Brassica napus (B. napus) mediated by Agrobacterium tumefaciens and compared these co-transformation methods. We tested a mixed-strain system, in which a sing

SUBMITTER: Liu F 

PROVIDER: S-EPMC7275470 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature