Biomarkers to quantify cell migration characteristics.

ABSTRACT:

Background

Because cell movement is primarily driven by the connection between F-actin and integrin through a physical linkage, cellular elasticity and adhesion strength have been considered as biomarkers of cell motility. However, a consistent set of biomarkers that indicate the potential for cell motility is still lacking.

Methods

In this work, we characterize a phenotype of cell migration in terms of cellular elasticity and adhesion strength, which reveals the interdependence of subcellular systems that mediate optimal cell migration.

Results

Stiff cells weakly adhered to the substrate revealed superior motility, while soft cell migration with strong adhesion was relatively inhibited. The spatial distribution and amount of F-actin and integrin were highly variable

SUBMITTER: Kwon S 

PROVIDER: S-EPMC7275471 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature