A new generation of recombinant polypeptides combines multiple protein domains for effective antimicrobial activity.

ABSTRACT:

Background

Although most of antimicrobial peptides (AMPs), being relatively short, are produced by chemical synthesis, several AMPs have been produced using recombinant technology. However, AMPs could be cytotoxic to the producer cell, and if small they can be easily degraded. The objective of this study was to produce a multidomain antimicrobial protein based on recombinant protein nanoclusters to increase the yield, stability and effectivity.

Results

A single antimicrobial polypeptide JAMF1 that combines three functional domains based on human α-defensin-5, human XII-A secreted phospholipase A2 (sPLA₂), and a gelsolin-based bacterial-binding domain along with two aggregation-seeding domains based on leucine zippers was successfully produced with no toxic effects

SUBMITTER: Roca-Pinilla R 

PROVIDER: S-EPMC7275485 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature