A new aggressive xenograft model of human colon cancer using cancer-associated fibroblasts.

ABSTRACT:

Background

Colorectal cancer is the second leading cause of cancer death. Almost half of the patients present recurrence within 5 years after the treatment of the primary tumor, the majority, with metastasis. On the other hand, in the search for new animal models that simulate metastatic cancer, it has been suggested that fibroblasts immersed in the peritumoral stroma (cancer-associated fibroblasts (CAFs)), play a relevant role in the development of cancer. The objective of this study was to identify an adequate animal model to study metastatic colon cancer and the application of new treatments.

Methods

Human CAFs and normal fibroblasts (NF) for transplant and culture were obtained from surgical fresh samples of patients with adenocarcinoma of sigmoid colon. Stromal cell pur

SUBMITTER: Fernando-Macias E 

PROVIDER: S-EPMC7275677 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature