Isolation, characterization, and cryopreservation of collared peccary skin-derived fibroblast cell lines.

ABSTRACT:

Background

Biobanking of cell lines is a promising tool of support for wildlife conservation. In particular, the ability to preserve fibroblast cell lines derived from collared peccaries is of significance as these wild mammals are unique to the Americas and play a large role in maintaining the ecosystem. We identified collared peccary fibroblasts by immunofluorescence and evaluated their morphology, growth and adherence capacity. Further, we monitored the viability and metabolic activity of the fibroblasts to determine the effects of passage number and cryopreservation on establishment of cell lines.

Methods

Skin biopsies were collected from the peripheral ear region from five adult animals in captivity. Initially, cells were isolated from fragments and cultured in the Dulb

SUBMITTER: Borges AA 

PROVIDER: S-EPMC7275682 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature