Reactive species generated by heme impair alveolar epithelial sodium channel function in acute respiratory distress syndrome.

ABSTRACT: We previously reported that the highly reactive cell-free heme (CFH) is increased in the plasma of patients with chronic lung injury and causes pulmonary edema in animal model of acute respiratory distress syndrome (ARDS) post inhalation of halogen gas. However, the mechanisms by which CFH causes pulmonary edema are unclear. Herein we report for the first time that CFH and chlorinated lipids (formed by the interaction of halogen gas, Cl₂, with plasmalogens) are increased in the plasma of patients exposed to Cl₂ gas. Ex vivo incubation of red blood cells (RBC) with halogenated lipids caused oxidative damage to RBC cytoskeletal protein spectrin, resulting in hemolysis and release of CFH. Patch clamp and short circuit current measurements revealed that CFH inhibited the

SUBMITTER: Aggarwal S 

PROVIDER: S-EPMC7276446 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature