Technical note: Relationship between placentome location and gene expression in bovine pregnancy.

ABSTRACT: A novel, non-terminal surgical procedure to remove a single placentome from the pregnant ewe for gene expression and histological analyses was recently developed in our laboratory. This technique allows for evaluation of nutritional insults on placental development at more than one stage of gestation using a single animal. Early attempts to develop a similar technique in cattle were met with complications due to inaccessibility of the gravid uterine horn because of its location and mass. One alternative is to collect a placentome from the contralateral uterine horn; however, the question remains as to whether gene expression varies among placentomes based on location relative to the fetus. Pregnant heifers were maintained on forage during early gestation and later moved into pens with a Ca

SUBMITTER: Picking EM 

PROVIDER: S-EPMC7276672 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature