Ontology highlight
ABSTRACT:
SUBMITTER: Sriramachandran AM
PROVIDER: S-EPMC7276987 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Molecular cell 20200421 5
DNA single-strand breaks (SSBs) are among the most common lesions in the genome, arising spontaneously and as intermediates of many DNA transactions. Nevertheless, in contrast to double-strand breaks (DSBs), their distribution in the genome has hardly been addressed in a meaningful way. We now present a technique based on genome-wide ligation of 3'-OH ends followed by sequencing (GLOE-Seq) and an associated computational pipeline designed for capturing SSBs but versatile enough to be applied to ...[more]