Antibody-Free Labeling of Malaria-Derived Extracellular Vesicles Using Flow Cytometry.

ABSTRACT: Extracellular vesicles (EVs) are cell-derived membrane-bound structures that are believed to play a major role in intercellular communication by allowing cells to exchange proteins and genetic cargo between them. In particular, pathogens, such as the malaria parasite Plasmodium (P.) falciparum, utilize EVs to promote their growth and to alter their host's response. Thus, better characterization of these secreted organelles will enhance our understanding of the cellular processes that govern EVs' biology and pathological functions. Here we present a method that utilizes a high-end flow cytometer system to characterize small EVs, i.e., with a diameter less than 200 nm. Using this method, we could evaluate different parasite-derived EV populations according to their distinct car

SUBMITTER: Dekel E 

PROVIDER: S-EPMC7277110 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature