Tailoring Uptake Efficacy of HSV-1 gD Derived Carrier Peptides.

ABSTRACT: Regions of the Herpes simplex virus-1 (HSV-1) glycoprotein D (gD) were chosen to design carrier peptides based on the known tertiary structure of the virus entry receptor complexes. These complexes consist of the following: HSV-1 gD-nectin-1 and HSV-1 gD-herpesvirus entry mediator (HVEM). Three sets of peptides were synthesised with sequences covering the (i) N-terminal HVEM- and nectin-1 binding region -5-42, (ii) the 181-216 medium region containing nectin-1 binding sequences and (iii) the C-terminal nectin-1 binding region 214-255. The carrier candidates were prepared with acetylated and 5(6)-carboxyfluorescein labelled N-termini. The peptides were chemically characterised and their conformational features in solution were also determined. In vitro int

SUBMITTER: Bosze S 

PROVIDER: S-EPMC7277387 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature