Different Impacts of MucR Binding to the babR and virB Promoters on Gene Expression in Brucella abortus 2308.

ABSTRACT: The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene babR encoding a LuxR-like regulator repressing virB genes. In this study, we show for the first time the ability of MucR to bind the promoter of babR in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate

SUBMITTER: Borriello G 

PROVIDER: S-EPMC7277663 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature