A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA.

ABSTRACT: Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of compounds. It is most promising to develop simple and effective biosensors for the use in situations where traditional methods are not available due to their complexity and laboriousness. In this project, a novel smart deoxyribozyme-based fluorescent sensor for the detection of androgen receptor mRNA was developed. It consists of several functional modules including two deoxyribozymes 10-23, an RNA-dependent split malachite green aptamer, and an oligonucleotide platform. Deoxyribozymes specifically release a 27-nucleotide RNA fragment that is rea

SUBMITTER: Bryushkova EA 

PROVIDER: S-EPMC7277777 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature