Analysis of trace metal distribution in plants with lab-based microscopic X-ray fluorescence imaging.

ABSTRACT:

Background

Many metals are essential for plants and humans. Knowledge of metal distribution in plant tissues in vivo contributes to the understanding of physiological mechanisms of metal uptake, accumulation and sequestration. For those studies, X-rays are a non-destructive tool, especially suited to study metals in plants.

Results

We present microfluorescence imaging of trace elements in living plants using a customized benchtop X-ray fluorescence machine. The system was optimized by additional detector shielding to minimize stray counts, and by a custom-made measuring chamber to ensure sample integrity. Protocols of data recording and analysis were optimised to minimise artefacts. We show that Zn distribution maps of whole leaves in high resolution are easily attainable in

SUBMITTER: Mijovilovich A 

PROVIDER: S-EPMC7278123 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature