Assessing the Performance of Dried-Blood-Spot DNA Extraction Methods in Next Generation Sequencing.

ABSTRACT: An increasing number of newborn screening laboratories in the United States and abroad are moving towards incorporating next-generation sequencing technology, or NGS, into routine screening, particularly for cystic fibrosis. As more programs utilize this technology for both cystic fibrosis and beyond, it is critical to identify appropriate DNA extraction methods that can be used with dried blood spots that will result in consistent, high-quality sequencing results. To provide comprehensive quality assurance and technical assistance to newborn screening laboratories wishing to incorporate NGS assays, CDC's Newborn Screening and Molecular Biology Branch designed a study to evaluate the performance of nine commercial or laboratory-developed DNA extraction methods that range from a highly puri

SUBMITTER: Hendrix MM 

PROVIDER: S-EPMC7278269 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature