Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle.

ABSTRACT: Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) proteins are recruited during CHIKV infection. Co-immunoprecipitation analyses revealed that both structural and nonstructural CHIKV proteins interact with he

SUBMITTER: Torii S 

PROVIDER: S-EPMC7278350 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature