Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs.

ABSTRACT: μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories in host cells, and the μNS protein has been suggested to be the minimal viral factor required for factory formation. Thus, determining the structure of μNS is of great importance for understanding its role in viral infection. In the study presented here, a fragment consisting of residues 448-605 of μNS was expressed as an EGFP fusion protein in Sf9 insect cells. EGFP-μNS_(448-605) crystallization in Sf9 cells was monitored and verified by several imaging techniques. Cells infected with the EGFP-μNS_(448-605) baculovirus formed rod-shaped microcrystals (5-15 µm in length) which were reconstituted in high-v

SUBMITTER: Nagaratnam N 

PROVIDER: S-EPMC7278499 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature