Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection.

ABSTRACT: Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix.

SUBMITTER: Peerzade SAMA 

PROVIDER: S-EPMC7279313 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature