Development of 3D Hepatic Constructs Within Polysaccharide-Based Scaffolds with Tunable Properties.

ABSTRACT: Organoids production is a key tool for in vitro studies of physiopathological conditions, drug-induced toxicity assays, and for a potential use in regenerative medicine. Hence, it prompted studies on hepatic organoids and liver regeneration. Numerous attempts to produce hepatic constructs had often limited success due to a lack of viability or functionality. Moreover, most products could not be translated for clinical studies. The aim of this study was to develop functional and viable hepatic constructs using a 3D porous scaffold with an adjustable structure, devoid of any animal component, that could also be used as an in vivo implantable system. We used a combination of pharmaceutical grade pullulan and dextran with different porogen formulations to form crosslinked scaffolds with macrop

SUBMITTER: Labour MN 

PROVIDER: S-EPMC7279349 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature