Regulation of I_Ca,L and force by PDEs in human-induced pluripotent stem cell-derived cardiomyocytes.

ABSTRACT:

Background and purpose

Phosphodiesterases (PDEs) are important regulators of β-adrenoceptor signalling in the heart. While PDE4 is the most important isoform that regulates I_Ca,L and force in rodent cardiomyocytes, the dominant isoform in adult human cardiomyocytes is PDE3.

Experimental approach

Given the potential of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for biomedical research, this study characterized the contribution of PDE3 and PDE4 isoforms to the regulation of I_Ca,L and force in hiPSC-CMs in an engineered heart tissue (EHT) model.

Key results

There was a lower abundance of mRNA for PDE3A and 4A in hiPSC-CM EHT than in non-failing human heart samples. Selective inhibition of PDE3 and 4 with cilostamide and r

SUBMITTER: Saleem U 

PROVIDER: S-EPMC7279982 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature