Implication of in vivo circulating fibrocytes ablation in experimental pulmonary hypertension murine model.

ABSTRACT:

Background and purpose

Recruitment and involvement of bone-/blood-derived circulating fibrocytes (CF) in the promotion of fibrotic tissue remodelling processes have been shown. However, their direct contribution to pathological changes is not clear. The present study investigates the causal role of CF in the pathogenesis of pulmonary hypertension (PH).

Experimental approach

For selective ablation of CF, we applied the suicidal gene strategy with herpes simplex virus thymidine kinase (HSV-TK) and ganciclovir. The transgenic mice were generated, having HSV-TK-GFP transgene under the collagen 1 promoter. To selectively target CF, HSV-TK-GFP⁺ bone marrow transplanted into irradiated wild type mice. These chimera mice were subjected to hypoxia for PH induction and ganc

SUBMITTER: Nikam VS 

PROVIDER: S-EPMC7279988 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature