A low MW inhibitor of CD44 dimerization for the treatment of glioblastoma.

ABSTRACT:

Background and purpose

As a hallmark of glioblastoma multiforme (GBM), CD44 plays a crucial role in promoting glioblastoma stem cell (GSC) stemness phenotypes and multiple drug resistance. The therapeutic potential of CD44 has been validated by the clinical successes of several CD44 inhibitors, including antibodies and hyaluronan-related drugs.

Experimental approach

We used systemsDock software to predict verbascoside as a candidate CD44 inhibitor. Microscale thermophoresis was used to confirm the interaction between CD44 and verbascoside. Four glioblastoma cell lines and a patient-derived glioblastoma cell line were used to test the influences of verbascoside on glioblastoma. CD44-overexpressing and CD44-knockout cell lines were also used. Real-time quantitative PCR and wes

SUBMITTER: Wang C 

PROVIDER: S-EPMC7280016 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature