Synovial fluid CD69⁺CD8⁺ T cells with tissue-resident phenotype mediate perforin-dependent citrullination in rheumatoid arthritis.

ABSTRACT:

Objectives

Although the importance of tissue-resident memory T (T_RM) cells in organ-specific chronic inflammation has been recognised, little is known about their role in rheumatoid arthritis (RA). Here, we examined the characteristics of synovial fluid CD8⁺ T cells that express canonical T_RM markers CD69 and CD103, and their role in the pathogenesis of RA.

Methods

Synovial fluid mononuclear cells (SFMCs) were obtained from patients with RA. Flow cytometric analysis of surface markers and cytotoxic molecules of CD8⁺ T cells was performed. TCR repertoire of CD8⁺ T cells was analysed by TCRVβ CDR3 sequencing. Citrullination with the formation of neutrophil extracellular trap (NET) was evaluated by immunofluorescence staini

SUBMITTER: Jung J 

PROVIDER: S-EPMC7280027 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature