Patterning of Particles and Live Cells at Single Cell Resolution.

ABSTRACT: The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or difficult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position,

SUBMITTER: Hacohen A 

PROVIDER: S-EPMC7281171 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature