Identification of Haemaphysalis longicornis Genes Differentially Expressed in Response to Babesia microti Infection.

ABSTRACT: Haemaphysalis longicornis is a tick and a vector of various pathogens, including the human pathogenetic Babesia microti. The objective of this study was to identify female H. longicornis genes differentially expressed in response to infection with B. microti Gray strain by using a suppression subtractive hybridization (SSH) procedure. A total of 302 randomly selected clones were sequenced and analyzed in the forward subtracted SSH cDNA library related to Babesia infection, and 110 clones in the reverse cDNA library. Gene ontology assignments and sequence analyses of tick sequences in the forward cDNA library showed that 14 genes were related to response to stimulus or/and immune system process, and 7 genes had the higher number of standardized sequences per kilobase (SPK). Subsequent real-

SUBMITTER: Zheng W 

PROVIDER: S-EPMC7281432 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature