Evaluation of Non-Uniform Sampling 2D ¹H-¹³C HSQC Spectra for Semi-Quantitative Metabolomics.

ABSTRACT: Metabolomics is the comprehensive study of metabolism, the biochemical processes that sustain life. By comparing metabolites between healthy and disease states, new insights into disease mechanisms can be uncovered. NMR is a powerful analytical method to detect and quantify metabolites. Standard one-dimensional (1D) ¹H-NMR metabolite profiling is informative but challenged by significant chemical shift overlap. Multi-dimensional NMR can increase resolution, but the required long acquisition times lead to limited throughput. Non-uniform sampling (NUS) is a well-accepted mode of acquiring multi-dimensional NMR data, enabling either reduced acquisition times or increased sensitivity in equivalent time. Despite these advantages, the technique is not widely applied to metabolomics. I

SUBMITTER: Zhang B 

PROVIDER: S-EPMC7281502 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature