Transcriptional Regulation of CCL2 by PARP1 Is a Driver for Invasiveness in Breast Cancer.

ABSTRACT:

Background

PolyADP ribosylation (PARylation) by PARP1 is a significant post-translational modification affecting protein function in various cancers. However, PARP1 mediated cellular processes in the context of breast cancer are not fully understood.

Method

To identify potential targets of PARP1, we carried out whole transcriptome sequencing with shRNA mediated PARP1 knockdown in triple-negative breast cancer (TNBC) cell line and inhibited PARP1 with a known PARP1 inhibitor, PJ34.

Results

Analysis of the transcriptomics data revealed that PARP1 is involved in regulating multiple chemokines under basal conditions, including the chemokine ligand 2 (CCL2). PARP1 knockdown and PJ34 mediated inhibition showed reduced CCL2 transcript levels in breast cancer ce

SUBMITTER: Dutta P 

PROVIDER: S-EPMC7281677 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature