Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells.

ABSTRACT: Neuronal development is a complex multistep process that shapes neurons by progressing though several typical stages, including axon outgrowth, dendrite formation, and synaptogenesis. Knowledge of the mechanisms of neuronal development is mostly derived from the study of animal models. Advances in stem cell technology now enable us to generate neurons from human induced pluripotent stem cells (iPSCs). Here we provide a mass spectrometry-based quantitative proteomic signature of human iPSC-derived neurons, i.e., iPSC-derived induced glutamatergic neurons and iPSC-derived motor neurons, throughout neuronal differentiation. Tandem mass tag 10-plex labeling was carried out to perform proteomic profiling of cells at different time points. Our analysis reveals significant expression changes (FDR

SUBMITTER: Varderidou-Minasian S 

PROVIDER: S-EPMC7281779 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature