Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter.

ABSTRACT:

Background

Thermostable enzymes are commonly produced in mesophilic hosts for research and bioengineering purposes. However, these hosts do not overexpress the active forms of some biologically functional thermoenzymes. Therefore, an efficient thermophilic expression system is needed. Thermus thermophilus contains an easily manipulable genome and is therefore among the best candidate microbes for a "hot" expression system. We previously identified a strong and inducible promoter that was active in T. thermophilus under supersaturated silica conditions. Here, we report a new heterologous gene expression system based on a silica-inducible promoter in T. thermophilus.

Results

A Thermus sp. A4 gene encoding thermostable β-galactosidase was cloned as a reporter gene into the expr

SUBMITTER: Fujino Y 

PROVIDER: S-EPMC7282064 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature