Effects of CO₂ limitation on the metabolism of Pseudoclostridium thermosuccinogenes.

ABSTRACT:

Background

Bio-based succinic acid holds promise as a sustainable platform chemical. Its production through microbial fermentation concurs with the fixation of CO₂, through the carboxylation of phosphoenolpyruvate. Here, we studied the effect of the available CO₂ on the metabolism of Pseudoclostridium thermosuccinogenes, the only known succinate producing thermophile. Batch cultivations in bioreactors sparged with 1 and 20% CO₂ were conducted that allowed us to carefully study the effect of CO₂ limitation.

Results

Formate yield was greatly reduced at low CO₂ concentrations, signifying a switch from pyruvate formate lyase (PFL) to pyruvate:ferredoxin oxidoreductase (PFOR) for acetyl-CoA formation. The corresponding increa

SUBMITTER: Koendjbiharie JG 

PROVIDER: S-EPMC7282089 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature